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GAP1 as an alternative counter-selectable marker in this system GAP1 is a native yeast gene encoding a general amino acid permease [39]

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This involves preparing samples and standards in a specific diluent, followed by incubation, washing, and detection steps that culminate in absorbance measurement at designated wavelengths

Last Resort: Tenex Procedure When conservative treatments fail to provide relief, tenex surgery may be considered as a last resort

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