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Epitalon Preparation Prof Khavinson (Saint Petersburg, Russia) kindly provided the tested peptide Epitalon, which was dissolved in water at a stock concentration of 100 g/mL, then further diluted to prepare the three concentrations for testing (20, 40, and 60 ng/mL)

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Injecting them within 60 minutes of a meal blunts growth hormone release by up to 80% (Nass et al., J Clin Endocrinol Metab, 2008)

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Briefly, 4 x lo5 spleen cells from aging (C57BW10 x DBN2)Fl mice were stimulated in culture with mitogens (concanavalin A, 0.5 p.g/well and phorbol myristate acetate, 4 ng/well) alone or with mitogens and THF-y2 or a given cytokine in various concentrations

Typical Concentrations Most cosmetic formulations contain 0.5%2% GHK-Cu