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Then we discard the culture medium and add 100 l CCK-8 solution (diluted with ECM to 10% concentration) into each well, and incubated the 96-well plates for 2 h, and the absorbance of the cells at 450 nm was measured by the multifunctional reader FlexStation 3 (Molecular Devices, CA, USA)

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Furthermore, exogenous administration of glucagon was shown early on to inhibit food intake in humans and rodents (62, 63)

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10.1017/cjn.2016.284 Can