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Gross et al., 2011

Yet, the startling reality is that metabolic and nutritional factors, many of which are modifiable, are now recognized as primary drivers of its pathogenesis, contributing to a global burden that afflicts over 300 million people (1)

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One study found that Prx3 (mitochondrial Prx) is highly oxidized in the cartilage of elderly patients with osteoarthritis, indicating that oxidative stress is intensified in degenerative cartilage

To quantify the protein content of patient biopsies, the Pierce bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific) was utilised, according to the manufacturers instructions

Before measurement, the samples were appropriately diluted with PBS at a ratio of 1:5 (v/v) 42,43