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Understanding whether B12 injections require refrigeration depends on the specific formulation

10.1007/BF00686159 Cancer Chemother

Kumar, H., Bhardwaj, K., Nepovimova, E., Kua, K., Dhanjal, D

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After drug treatments, the cells were fixed with 4% paraformaldehyde for 20 min and infiltrated with 0.2% Triton X-100 for 15 min, followed by blocking in donkey serum for 30 min and incubating overnight at 4C with primary antibody (GPX4, rabbit, ab125066, 1:200, Abcam) in PBS