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After pharmacological intervention in 96-well cell culture plates for 24, 48 or 72 h, the culture medium was removed and replaced with 0.1 mg MTT reagent (Beyotime, ST1537) per well dissociated in 100 L basal DMEM (Procell, PM150210)
These findings suggest that excessive cell death and oxidative stress were common characteristics between granulosa cells and oocytes in hypothyroidism
We use thigh muscle for ease when sitting down
Antioxidants and mitochondrial fission inhibitors may serve as adjunctive therapeutic options
