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Interestingly, a copper-dependent loss of AP1 activity was observed after 24 h in HepG2 ATP7B-KO cells at higher concentrations

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Furthermore, unlike fructose, allulose is not a substrate for de novo lipogenesis and elicits no inhibitory effect on fatty acid oxidation [1]

In fact, it may even act as a pro-oxidant in some cases

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Use of broad-spectrum antibiotics may also raise the risk of deficiency, as well as very high doses of vitamin A, which seem to reduce vitamin K absorption