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Resveratrol and lycium barbarum polysaccharide improve Qinling giant panda (Ailuropoda melanoleuca Qinlingensis) sperm quality during cryopreservation

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The diluted lysates were incubated with 2 l of GFP antibody (Proteintech, PABG1) overnight at 4C on rotation and for two additional hours with mixed Dynabeads protein G and A (15:15 l, Life Technologies)

Plasmid construction, transfection, RNA interference (RNAi) For overexpression studies, Sororin and RSMC cDNA were cloned into the pRK5-Flag, Myc, and pEGFP-C1 mammalian expression vectors, respectively

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