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Lacy, Chemokine pathways in cutaneous melanoma: their modulation by cancer and exploitation by the clinician

Neuroscience Letters, 583, 81-87

found that GHK-Cu stimulated collagen synthesis by fibroblasts, noting that the presence of a GHK triplet in type I collagen meant that GHK may offer in situ healing effects [1, 6]

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The sections were air-dried, washed three times for 5 min each in 0.3% Triton X-100 in PBS (PBS-Triton), and then blocked with 1% bovine serum albumin (BSA) in PBS for 2 h at room temperature