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The very low rate constants for direct, glutathione-independent dithiol-disulfide exchange reactions between glutaredoxins and roGFP2 or other protein(S 2 )/protein(SH) 2 couples (despite favorable thermodynamics) are a prerequisite and great advantage for reliable redox measurements in vivo

TUNEL assay The death cells in mice lung sections were measured by TUNEL Apoptosis Detection Kit (YEASEN,40307ES60) according to the manufacturers instructions

If it keeps happening, the question shifts from How do I calm this down today? to Why is this still happening at all? That shift is important

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