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Selective Suppression in Mixed Cultures: Incorporated into media to inhibit susceptible bacteria while maintaining resistant strains
Cell-Based Luciferase Reporter Assays
It is reduced to NADH (Cat# N-035) which carries electrons to the electron transport chain for subsequent oxidative phorphorylation and ATP production
How It Works: Expression of the SAT1 gene provides resistance to nourseothricin
0 at 25°C using DNA
Bluo-Gal T330 Selective Suppression in Mixed Cultures:Bluo Gal is used as an alternative to X Gal, and produces a darker blue color. Bluo Gal is hydrolyzed by galactosidase to produce a blue precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial colonies containing active galactosidase produce blue colonies when grown on plates containing Bluo Gal. Bluo Gal is commonly used in conjunction with IPTG (GoldBIo
