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Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications (8th ed
NaCl) or EDTA to make buffers like TBS (Tris-buffered saline) and TBST (Tris-buffered saline + Tween) used in immunoassays
Nigericin sodium disrupts membrane potential by acting as an antiporter of H+ and K+ ions by exchanging K+ for H+
Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids
and protein tyrosine kinase assays
n-Dodecyl-B-D-maltoside (DDM) D133 Culture of Animal Cells: An Dodecyl B D Maltoside, or DDM, is a maltoside based non ionic detergent with a hydrophilic maltose head and a hydrophobic long chain alkyl tail. It is considered a gentle detergent that is more efficient than other detergents, such as CHAPS or NP 40. It has a comparatively low critical micelle concentration (CMC) of 0. 17mM compared to the other maltoside based detergents. The primary attribute of DDM is its capability to extract hydrophobic
