Wang J., Chen J., Tang Z., Li Y., Hu L., and Pan J., The Effects of Copper on Brain Microvascular Endothelial Cells and Claudin via Apoptosis and Oxidative Stress, Biological Trace Element Research 174 (2016): 132141, 10.1007/s12011-016-0685-4
3.2 Physical properties of bee venom BV is a translucent liquid with a pH range of 4.5 to 5.5, characterized by an unpleasant taste and odor
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Due to this, citric acid is usually included in small amounts as a pH adjuster
Trypsin digests were diluted with water
Protein concentrations were determined using the Bio-Rad protein assay (Bio-Rad, CA, USA) based on the Bradford method ( ASSAYS OF GGT ENZYME ACTIVITIES Assays of GGT enzyme activities were performed by analyzing the amount of deglutamylated compounds produced from -glutamylated compounds by yeast crude proteins in 6 h at 37C