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42 , 43 These clinical genetics studies also calculated that individual environmental factors contributed 26% to 37% and age 11% to 16% toward the causation of cortical cataracts.

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As the medium is usually high in B9 (about 9 mM), we simulated a state of nutrient inadequacy by utilizing a depleted medium with reduced levels of vitamin B9 sourced only from the added serum (34)

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Of those, 35 were preclinical (animal studies) and one was clinical (human)