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Libraries were enriched and indexed using 12 cycles of amplification (LongAmp Taq 2 MasterMix, New England BioLabs Inc.) with PCR primers which include a 6 bp index sequence to allow for multiplexing (custom oligo order from Integrated DNA Technologies)

[1] Of the 15% of revenue that is directly credited to the general fund, the state treasurer must transfer the total revenue attributable to the new nicotine products tax and the tax increases on tobacco products, modified risk tobacco products, and moist snuff, to the 2020 Tax Holding Fund, created by the measure

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