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US Surgeon General, 2004)

Measurement of protein levels of FoxA2 and E-cadherin by Western blotting Protein extracts from cells and tissue were mixed with 5 sample loading buffer, boiled at 100C for 10 minutes, centrifuged, and cooled to room temperature

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The absorbance was measured at 535 nm (e 20500 M -1 cm -1 )

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