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PMID 30623881

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For a display of this size 14 by 11.4 inches a standard frame size in the 16x20 range accommodates a generous mat border

Wang H, Naghavi M, Allen C, et al

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The maximal OCR is attained by adding the uncoupler Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP), which mimics a physiological energy demand by stimulating the respiratory chain to operate at maximum capacity and causing the rapid oxidation of the substrates (sugars, fats, amino acids) to meet the metabolic challenge