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The same amount of protein was immunoprecipitated with HIF-1 antibody, and Western blot analysis of immunoprecipitates with acetyllysine antibody was performed

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The first aim of this project is to establish this model by exposing Scnn1b -overexpresssing and wild type airway epithelia to tobacco or sham smoke and analyzing the following parameters related to disease: (i) pulmonary immune cell populations and cytokine production