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This result can be partially explained by the fact that the half-life of cotinine is ~17 hours in human and ~1 hour in mice 7,22
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On the sixth and the seventh days of cigarette smoke exposure (i.e., on the two days preceding the behavioural tests), the experimenter handled each animal for 5 min to avoid any handling stress on the test day
Additionally, roll-your-own kits may seem cost-effective but often lead to increased consumption
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