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The FDA proposal is part of an effort to strengthen the agency's ability to identify illegal foreign tobacco products

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Spoon a regular teaspoonful (0.4 to 0.5 ounce) of paste into the center of the stencil

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After deparaffinization, antigen retrieval and permeabilization, the samples were blocked and probed with the rabbit polyclonal antibodies against loricrin, involucrin (both Abcam, UK), filaggrin, KLK7 and TG1 (all three Invitrogen, USA) and appropriate anti-rabbit secondary antibody conjugated with Alexa Fluor 555 (Invitrogen, USA)