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Supernatant was transferred to 1.5 mL microcentrifuge tube after which the remining pellet was resuspended in 400 L 20 C 40:40:20 Acetonitrile (ACN): MeOH: H2O extraction solvent and incubated on ice for 5 minutes

Most pharmacies use 10 mg/mL or higher to keep volumes under 1 mL per dose

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Intracranial injections were performed under deep anaesthesia using vaporized isoflurane (22.5%) and oxygen (2 l min 1 )

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