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Their structure is composed of four domains (Figure 2) [26, 30, 46, 47]: An amino-terminal arm An anti-parallel eight-stranded -barrel domain Wrapping domain -helical domain The amino-terminal domain is an extended arm and is quite variable in length ranging from 53 residues in catalase (PMC) to 127 in HPII [30, 47]

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Purification of GST-tagged proteins