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Hematoxylin and eosin were used to stain a 45 m-thick section for histological examination (H&E) ( 2.8 Immunohistochemical staining Following the protocol, immunohistochemical labelling of specific liver sections from both the control and treatment groups was conducted to assess caspase-3 as a marker of apoptosis using avidin-biotin-peroxidase (DAB, Sigma Chemical Co.) ( ) stain
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Problems can occur when inflammatory signaling becomes persistent, poorly regulated, or disconnected from the original trigger
