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During the entire experiment, the mice were housed under rigorously controlled conditions, including a temperature of 23C 2C, a relative humidity of 45% 5%, and a consistent 12-hour light/dark cycle

10.1038/s41598-020-77578-x 119

We make micro-infusion tools, including the Under-Eye MicroInfuser patches and the upcoming MicroInfuser stamping device

6 weeks (200300 mcg/day): ~2 vials 8 weeks (200300 mcg/day): ~3 vials Advanced (250 mcg, 2/day): ~6 vials Insulin Syringes (U-100, 1 mL) Per injection: 1 syringe 8 weeks (once daily): ~56 syringes 8 weeks (twice daily): ~112 syringes Bacteriostatic Water (10 mL bottles) Use 3.0 mL (standard) or 2.0 mL (advanced) per 5 mg vial for reconstitution

References Barisic I, Balenovic D, Klicek R, Radic B, Nikitovic B, Drmic D, Udovicic M, Strinic D, Bardak D, Berkopic L, Djuzel V (2013) Mortal hyperkalemia disturbances in rats are NO-system related

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