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Finally, sections were observed using a Leica DM4000 B epifluorescence microscope

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Another study indicated that C57BL/6 mice with neuropathic pain, treated intraperitoneally with repeated doses of 10 mg/kg SFN, exhibited reduced anxiety- and depressive-like behaviors associated with chronic neuropathic pain (79)

Mice were anesthetized by inhaling a mixture of 1 L/min oxygen and 1%1.5% isoflurane via a mask, while maintaining normal respiration

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