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For total ATP content measurements, cells were lysed with CellTiter-Glo Reagent (Promega Corporation, Madison, WI, USA) and total ATP determined

Samples were then transferred to 0.2 m centrifugal filters and centrifuged again at 10,000 RCF for 5 min at 4C before being transferred to glass auto-sampler vials ready for injection

Being sleep-deprived wears on your body and increases the damage from oxidative stress, which means your natural glutathione is used to defend your body from this stress, reducing your levels

All authors approved the final version of the manuscript for submission

10.2337/diab.23.3.247 208 LegouisD.FaivreA.CippaP

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