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GSH or Ni-NTA bead washing was performed four times with cold PBS or washing buffer (20 mM Tris-Cl (pH 8.0), 300 mM NaCl, 20 mM imidazole)

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doi: 10.1371/journal.pone.0226999 122 LopesFNCda CunhaNVde CamposBHFattoriVPanisCCecchiniRet al

However, free radicals generated as a result of metabolic events can accumulate in the body and lead to the deterioration of the egg or sperm

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