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After treatment, cells were harvested and incubated in a buffer containing FITC Annexin V and propidium iodide (556547, BD Pharmingen) for 15 min at room temperature (25 C) in the dark, followed by flow cytometric analysis

All cervical lymph nodes should be palpated in case carcinoma is already present and has spread to involve regional lymph nodes

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Dave Navarro DAVE NAVARRO STROLLING ON SUNSET BOULEVARD IN LOS ANGELES ON SEPTEMBER 28, 2005

Did that actually happen