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Our hypothesis is that GPx1 concentration in breast cells is controlled by (i) factors that affect Se bioavailability (Se supply and polymorphisms in SEPP1) or the selenoprotein hierarchy (e.g

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Gut 64 , 11481157 (2015)

At 2 h after plating, the plating medium was replaced with fresh culture medium consisting of DMEM supplemented with 1 mM sodium pyruvate, 100 units/ml penicillin, 100 g/ml streptomycin, 0.2 % (vol/vol) of bovine serum albumin fraction V (instead of FBS), 100 nM dexamethasone, and 1 nM insulin (SigmaAldrich #I9278)

PMCID: PMC2696075.