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Passage 1 or 2 cultures were seeded onto collagen IV (C7521, Sigma, St Louis, MO) coated 12 mm Transwell inserts (3460, Corning Costar Corporation, Cambridge, MA) and grown in ALI media to 80% confluence before exposing the apical surface to air

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They hold a significant place in tobacco culture for several reasons: Accessibility: Widely available in almost any corner store, they are incredibly easy to acquire

Foreign laws differ greatly from place to place

doi: 10.1300/J233v04n03_01

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