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Briefly describing, 450 l phosphate buffer was added to 50 l supernatant and was subject to ultrafiltration (ULTRAFREE-0.5 Centrifugal Filter Device (UFV5BGC100), Millipore, MA, USA) at 12000 g at 4C for 60 min

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however, the inhibition of rebound contractions was not mediated via CB1, CB2, or TRPV1 [78]

hTAAR1 EC50 values (1016 M) were within the clinically observed therapeutic solriamfetol plasma concentration range and overlapped with the observed DAT/NET inhibitory potencies of solriamfetol in vitro

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