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This disparity created problems for Formula 1, which had to manage a patchwork of regulations depending on where the race was held

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Following 24 hours total RNA was extracted from exposed to CS and TGF- treated with NHBE ALI cultures by using the Qiagen RNeasy mini kit (Cat # 74104) and cDNA was reverse transcribed by the Applied Biosystems TaqMan Advanced miRNA cDNA Synthesis Kit (Life Technologies/Applied Biosystems, Cat # A28007) according to the manufacturers instructions

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