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We determined the copy number by using the following formula: and the following link was used to calculate the final copy number: Generation and analysis of the bulk-RNA-seq data Total RNA was isolated from cells using RNeasy Plus Mini kit (Qiagen) according to manufacturers instructions

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Sperm in smokers may show more abnormalities affecting fertility

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nicotine sprays and patches Nicotine sprays are sprayed into the mouth 1 to 2 times per hour