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The mortality rates (MR) for never, current and former smokers for age group x were then converted into the probability of dying for age group x using the formula: Rights and permissions Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made

He described himself as incapable of nurturing friendships

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After exposure, cells were fixed with 4% paraformaldehyde for 10 min, then stained with 1x Phalloidin-iFluor 594 ab176757 (Abcam, Cambridge, United Kingdom) for 1 h, following the assay procedure recommended by the manufacturer

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