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To understand the underlying molecular mechanisms, the expression levels of 3-AR mRNA in both white and brown adipose tissue were assessed using reverse transcription followed by PCR and Southern blotting

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Freeze-thaw sensitivity is lower than for a peptide, since there is no secondary structure to lose, but aliquoting still avoids repeated stopper punctures and concentration drift from evaporation

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Having a metabolic disease such as obesity or type 2 diabetes, as well as a low-choline, methionine, or inositol diet, can make weight loss difficult