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The animal model showed significant decrease in DAI, biochemical markers, inflammatory scores including 8-hydroxyguanosine and nitrotyrosine in colon mucosa which were aggravated by DSS administration

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The loss of TH-positive neurons caused by MPTP and improvement by administration of the supplemented diets was confirmed with Nissl staining (Nissl positive cells) for counting the loss of total neurons in the SNpc (Figure 1B)

The entire procedure typically takes around 20 to 30 minutes, depending on the size and number of areas being treated

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