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Following coating, NCI H716 cells were seeded at a concentration of 1 10 5 cells/well in Dulbeccos Modified Eagles Medium (DMEM) supplemented with 10% (v/v) NBCS, as determined by trypan blue staining using a hemocytometer

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Acute hemolysis from G6PD deficiency is linked to the development of Heinz bodies in red blood cells (dark brown spots one indicated by the white arrow in the figure above), which are composed of denatured and aggregated hemoglobin